近日，真邁生物(wù)自主研發的(de)創新技術再次獲得(de)國際認可(kě)，包括全内反射熒光(guāng)成像系統在内的(de)三項發明(míng)專利正式通(tōng)過歐洲專利局（European Patent Office）的(de)授權批準，成爲有效歐洲專利。
至此，曆經多(duō)年自主研發積累，真邁生物(wù)目前擁有的(de)國内外專利（含授權有效和(hé)申請中）及計算(suàn)機軟件著作權登記等已近200件。
01
METHOD FOR CONTROLLING BASE SEQUENCE DETERMINATION REACTION, BASE SEQUENCE DETERMINATION SYSTEM AND CONTROL DEVICE
（對(duì)序列測定反應進行控制的(de)方法、序列測定系統和(hé)控制裝置）
技術簡介：
序列測定系統包括流體裝置和(hé)光(guāng)學裝置，反應裝置包括第一單元和(hé)第二單元，定義序列測定反應包含的(de)一種重複執行單位爲第二生化(huà)反應-第一生化(huà)反應-拍(pāi)照(zhào)，方法包括在完成初始步驟之後，使得(de)當利用(yòng)流體裝置使第一單元和(hé)第二單元中的(de)一個(gè)上的(de)樣品進行第二生化(huà)反應和(hé)第一生化(huà)反應的(de)同時(shí)，利用(yòng)光(guāng)學裝置對(duì)另一個(gè)單元上的(de)樣品進行拍(pāi)照(zhào)。初始步驟包括：a）利用(yòng)流體裝置使第一單元和(hé)第二單元中的(de)一個(gè)進行樣品的(de)第一生化(huà)反應，b）利用(yòng)光(guāng)學裝置對(duì)進行第一生化(huà)反應後的(de)單元的(de)樣品的(de)進行拍(pāi)照(zhào)，c）利用(yòng)流體裝置使第一單元和(hé)第二單元的(de)另一個(gè)進行樣品的(de)第一生化(huà)反應。上述方法可(kě)提高(gāo)序列測定效率。
“The present disclosure discloses a method for controlling a base sequence determination, a base sequence determination system, and a control device. The base sequence determination system includes a fluid device and an optical device, a reaction device includes a first component and a second component, and a repeated executable unit included in the base sequence determination is defined as: a second biochemical reaction—a first biochemical reaction—photographing. The method includes, after initiation steps are completed, using the fluid device to perform the second biochemical reaction and the first biochemical reaction of the sample on the first component, while using the optical device to photograph the sample on the second component. The initial steps include: a. using the fluid device to perform the first biochemical reaction of the sample on the first component, b. using the optical device to photograph the sample on the first component after the first biochemical reaction, and c. using the fluid device to perform the first biochemical reaction of the sample on the second component. The above-mentioned method can improve the efficiency of base sequence determination.”
02
SINGLE-MOLECULE IMAGE CORRECTION METHOD, DEVICE AND SYSTEM, AND COMPUTER-READABLE STORAGE MEDIUM
（單分(fēn)子圖像糾偏方法及裝置）
技術簡介：
本專利公開了(le)一種單分(fēn)子圖像糾偏方法及裝置，其中方法包括：根據第一圖像的(de)每一像素點對(duì)應的(de)亮度值矩陣，獲取與所述第一圖像對(duì)應的(de)第一目标簡化(huà)矩陣，并根據第二圖像的(de)每一像素點對(duì)應的(de)亮度值矩陣，獲取與所述第二圖像對(duì)應的(de)第二目标簡化(huà)矩陣；對(duì)所述第一目标簡化(huà)矩陣和(hé)所述第二目标簡化(huà)矩陣分(fēn)别進行二維離散傅裏葉變換，得(de)到與所述第一目标簡化(huà)矩陣對(duì)應的(de)第一傅裏葉矩陣，并得(de)到與所述第二目标簡化(huà)矩陣對(duì)應的(de)第二傅裏葉矩陣；根據所述第一傅裏葉矩陣和(hé)所述第二傅裏葉矩陣獲取所述第二圖像相對(duì)于所述第一圖像的(de)偏移量；根據所述偏移量對(duì)所述第二圖像進行糾偏。可(kě)見，實施本發明(míng)能夠對(duì)不同時(shí)刻拍(pāi)攝的(de)單分(fēn)子圖片進行糾偏。
“An embodiment of the present disclosure discloses a method and a device of correcting a single-molecule image, the method includes: acquiring the first target simplified matrix corresponding to the first image according to the intensity matrix corresponding to each of pixels in the first image, and acquiring the second target simplified matrix corresponding to the second image according to the intensity matrix corresponding to each of the pixels in the second image; the two-dimensional DFT is performed to the first target simplified matrix and the second target simplified matrix respectively to acquire the first Fourier matrix corresponding to the first target simplified matrix and the second Fourier matrix corresponding to the second target simplified matrix; the method of correcting a single-molecule image acquire the offset of the second image with respect to the first image according to the first Fourier matrix and the second Fourier matrix; and correcting the second image according to the offset. Such method can correct the images of single molecules taken at different moments.”
03
TOTAL INTERNAL REFLECTION FLUORESCENCE IMAGING SYSTEMAND SEQUENCING DEVICE
（全内反射熒光(guāng)成像系統及測序裝置）
技術簡介：
本專利公開了(le)一種全内反射熒光(guāng)成像系統及測序裝置，全内反射熒光(guāng)成像系統包括第一成像系統和(hé)第二成像系統，所述第一成像系統包括N條獨立的(de)激光(guāng)光(guāng)路，所述第二成像系統通(tōng)過第二接收裝置獲取到第二像的(de)位置的(de)不同判斷樣品高(gāo)度的(de)變化(huà)，根據樣品高(gāo)度的(de)變化(huà)進行自動補償，以保證樣品始終處于聚焦的(de)位置。本發明(míng)可(kě)通(tōng)過第二成像系統分(fēn)别對(duì)N條獨立的(de)激光(guāng)光(guāng)路進行光(guāng)路調節，以實現N條獨立的(de)激光(guāng)光(guāng)路具有相同的(de)穿透深度，進而無需在切換不同光(guāng)源時(shí)，再進行光(guāng)路調節，從而節省了(le)實驗當中費時(shí)的(de)光(guāng)路調節。
“The present disclosure discloses a total internal reflection fluorescence imaging system and a sequencing device. The total internal reflection fluorescence imaging system includes a first imaging system and a second imaging system. The first imaging system includes N separate laser light paths. The second imaging system determines the changes of the height of the sample by means of the differences of the positions at which the second receiving device acquires the second images, and performs automatic compensation according to the changes of the height of the sample, so as to ensure that the sample is always at a focused position. The embodiment can perform light path adjustment of the N separate laser light paths respectively by means of the second imaging system, so as to realize that the N separate laser light paths have the same penetration depth. Therefore no further light path adjustment is required when switching between different light sources, thus saving the time consuming for light path adjustment.”
自主研發、科技創新是真邁創立之初的(de)内在基因，也(yě)是真邁持續發展至今的(de)戰略根基。爲了(le)有效支撐公司科技創新戰略，包括有效地對(duì)無形資産進行轉化(huà)和(hé)管理(lǐ)運營，公司一直秉承尊重科學家和(hé)工程師的(de)團隊文化(huà)，并據自身情況設立專門部門和(hé)人(rén)員(yuán)進行知識産權管理(lǐ)，公司知識産權的(de)管理(lǐ)運行符合國家推薦性标準GB/T 29490-2013《企業知識産權管理(lǐ)規範》。未來(lái)，真邁将進一步加強深化(huà)知識産權布局，持續促進公司核心競争力的(de)穩步提升。